Antigen

Antigen Class

TFs and others

Antigen

ATF1

Cell type

Cell type Class

Muscle

Cell type

DTC1

NA

NA

Sample

source_name

Cell line

cell type

Derived from malignant pleural effusion of a patient with a chest wall CCS tumor

chip antibody

ATF1 C (LS-C351857, LS Bio)

treatment

Transfected with siRNA targeting EWSR1-ATF1 fusion transcript

cell line

DTC1 (CVCL_0J31)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked with formaldehyde, flash-frozen and processed for ChIP using Protein G beads. ChIP DNA was purified with AMPure XP beads. ChIP DNA libraries were prepared using the Illumina TruSeq ChIP library preparation kit (Illumina cat. no. IP-202-1012 or IP-202-1024) according to the manufacturer's instructions and 200-500 bp fragments selected using AMPureXP beads.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

31962270

Reads aligned (%)

98.5

Duplicates removed (%)

27.2

Number of peaks

9445 (qval < 1E-05)

hg19

Number of total reads

31962270

Reads aligned (%)

97.5

Duplicates removed (%)

28.7

Number of peaks

9331 (qval < 1E-05)